Bioassay of cytokinin

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August undefined, 2024

WebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been … WebA bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: …

Isolation and assay of a cytokinin from barley - ScienceDirect

WebThe induction of shoot buds from the filamentous protonema of moss is a classic bioassay for cytokinin. While a large literature documents this response in many species of moss and for a wide range of natural and synthetic cytokinins, to date only substituted adenine cytokinins have been examined in detail. This paper shows that at least some ... Weband the soybean callus bioassay. The highest con-centration of cytokinin was DHZR at 35.6 ug 1-1 followed by iP 15.9 jig 1-1. The cytokinin with the lowest concentration was tZ at 0.7 pg 1-1. The total cytokinin concentration in Seasol was 0.115 mg 1-1 (Tay et al., 1985). Zeatin-O-glucoside (ZOG) and how are pension distribution taxed

Laboratory protocols for the quantitation of cytokines by …

WebJan 1, 1989 · The response of the Biosynthesis of cytokinins by A. chroococcum 969 sample observed in the bioassay was compared to the response observed by a known concentration of r-zeatin. The amount of cytokinin-like compounds present was expressed in terms of the concentration of zeatin equivalents (ZE). WebCytokinin travels from the roots into the shoots thereby signaling bud growth in length. Cytokinin has shown the slow aging of plant organs by preventing breakdown of … WebOct 2, 2016 · Prof. Bhagwan Deen. Physiological role: The cytokinin checks the degradation of chlorophyll and increases the RNA, DNA and protein content. It plays following role in plants: Cell division: Cytokinin promotes cell division in plant tissues in the presence of auxins. It promotes cell division in apical meristematic and cambium tissues … how are people affected by war

Biological activity of cytokinins derived from Ortho- and …

Phenylurea cytokinins assayed for induction of shoot buds in

WebNaturally occurring cytokinins are adenine-based plant hormones. Although, the effect of various substituents at positions N1, C2, N3, N 6, N7, or N9 on the biological activity of cytokinins has been studied, the C8-substituted compounds have received little attention.Here, we report the synthesis and in vitro biological testing of thirty-one … WebThe activity, uptake and metabolism of cytokinin metabolites was determined in soybean (Glycine max (L.) Merr.) callus tissue. The following activity sequence was established: zeatin riboside (ZR)>zeatin (Z)>O-glucosides of Z, ZR and their dihydro derivatives>lupinic acid (an alanine conjugate of Z)>7- and 9-glucosides of Z which were almost inactive. how many miles an hour can usain bolt run how are people being scammed with zelle

"WebJan 7, 2009 · Miller (1956) identified the first cytokinin, called kinetin, in the herring sperm. Cytokinins occur in most plants including mosses, ferns, conifers, algae and diatoms; II. Chemistry ... This is the basis for the classical bioassay. The mechanism is associated with increased plasticity of the cell wall, not associated with acidification. " - Bioassay of cytokinin

Bioassay of cytokinin

Isolation and assay of a cytokinin from barley - ScienceDirect

WebNational Center for Biotechnology Information WebJul 23, 2024 · Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S …

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WebWe also report here an improved chlorophyll retention bioassay based on incubation of 2.5 cm long detached wheat leaf segments in microtiter plate wells containing 150 µl of … Webkinin bioassay. Cytokinin-like activity was determined by the radish cotyledon assay. The radish cotyledon bioassay. Radish seeds, Raphanus sativus L. cv. Okura Daikon, were germinated in darkness at 27°C on wet filter paper in plastic boxes. After about 3 days, larger cotyledons were excised from each

WebFeb 1, 1998 · The purpose of these protocols is to provide practical stepwise methods for the bioassay of cytokines using cytokine responsive cell lines. They include tables of the … WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2-chloro-6-(3 …

WebMar 1, 1974 · Cucumber cotyledon expanSIon in dark can therefore serve as a bioassay system for cytokinins. A comparison of the activity of different concentrations of kinetin and benzylaminopurine on cotyledon expansion in dark is shown in table 6 and figs. 3 to 5. It will be seen that benzylaminopurine is more active than kinetin in cucumber cotyledons ... WebA rapid bioassay for cytokinins is described. This is based on the ability of these compounds to promote markedly the expansion of radish cotyledons excised soon after …

WebNov 1, 2015 · Abstract. The cytokinins have been implicated in many facets of plant growth and development including cell division and differentiation, shoot and root growth, apical dominance, senescence, fruit and seed development, and the response to biotic and abiotic stressors. Cytokinin levels are regulated by a balance between biosynthesis [isopentenyl ...

WebMay 1, 1972 · The cytokinin bioassay based upon these two effects is simple and provides results after a relatively short time. The root length is measured after 6 days of … how many miles an hour can a cheetah runWebHer primary areas of investigation include Botany, Cytokinin, Food science, Horticulture and Shoot. Her study in Botany is interdisciplinary in nature, drawing from both Micropropagation and Tissue culture. Her work in the fields of Zeatin overlaps with other areas such as Seedling, Germination, Bioassay and Callus. how are pension contributions worked outWebXanthium expresses a large and rapid cytokinin response which can be obtained in very small solution volumes, and which is sensitive to cytokinin concen-trations as low as 10 … how many miles are 11 000 stepsWebJul 1, 1992 · The bioassay is based on the expression in transformed tobacco ( Nicotiana tabacum) mesophyll protoplasts of a chimeric gene, consisting of the upstream … how are people affected by air pollutionWebMar 1, 2009 · The development of sensitive analytical methods for the determination of cytokinin levels in plant tissues is essential for elucidating the roles of cytokinins in life science. In the past decade ... how are people baptizedWebMay 15, 2013 · In a bioassay the activity of a sample is tested on a sensitive cell line and the results of this activity are compared to a standard cytokine preparation. This assay is … how are people affording carsWebCytokinin is synthesized in the roots from where they are transported to shoots by xylem tissues. Biosynthesis of purine type of cytokinin is more clearly known than of other types. Various components of purine are derived from several small molecules including formyltetrahydro folate, aspartate, CO 2, ... how are pension calculated