WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably … WebFeb 11, 2024 · Results: A range of sialyltransferase from mammals, birds and fish were stably expressed in CHO Flp-In cells. The stable cell lines expressing ST6Gal1 modify the glycans on the surface of the CHO cells as detected by fluorescently labelled lectin microscopy. The catalytic domains, as isolated by Ni Sepharose from culture media, …
Establishment of Flp-InTM CHO cell lines with double expression …
WebInvitrogen™ Flp-In™-CHO Cell Line. Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector. Includes: 3 x 10 6 cells are … WebDec 1, 2024 · Flp-In-CHO cells (Life Technologies) were maintained in F12 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco) at 37 °C in a humidified incubator containing 5% CO 2. The cells were transfected with 1 μg plasmid (pDF-λ1:pOG44 was 1:9) using Lipo 2000 (Life Technologies) according to the … how to split a vector in c++
[Establishment of Flp-InTM CHO cell lines with double …
WebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each WebMar 22, 2024 · Cystic fibrosis (CF) is a genetic disease that affects the lungs and digestive system by making fluids and mucus thick and sticky. This causes persistent lung infections that progressively limit the ability to breathe. Children with CF also suffer from digestive problems that prevent their bodies from breaking down and absorbing food, leading ... WebNov 11, 2013 · Flip-In CHO cells (FCHO) stably transfected by plasmid DNA were stained by PE-conjugated mouse-anti-human kappa chain antibodies (PE-Kappa) and then analyzed by FACS. ... Invitrogen (2006) Flp-In system for generating stable mammalian expression cell lines by Flp recombinase-mediated integration. Instruction Manual. Carlsbad, CA. 22. rea english 6-12