Fluxor herg assay h9c2

Author: fjrl

August undefined, 2024

Web• The non-homogeneous potassium ion channel assay was run using FluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturer’s protocol. • All plates were run on FLIPR Tetra Systems using the Calcium Assay setup (Ex 485nm, Em 530nm). Data were acquired at 1 - or 2-second intervals for 140 to 200 seconds. Figure 2. K. V WebHere, we sought to develop an optical high-throughput assay to monitor surface and total hERG protein expression that would be useful to discern mechanisms underlying LQT2 …

FluxOR Potassium Ion Channel Assay J1 13Aug2009

WebJul 16, 2024 · H9C2 cells were subcultured under anoxic conditions to mimic myocardial hypoxia and subsequently treated with salidroside. Whole cell patch clamp was … WebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and … how far is george from knysna

FluxOR Potassium Ion Channel Assay - Thermo Fisher Scientific

WebFluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium … WebThis also means that the assay is carried out in a physiological, normal-chloride saline. in this article, the authors describe how they used Bacmam gene delivery to express Kv7.2 and 7.3 (KcnQ), Kir2.1, or Kv11.1 (herg) potassium ion channels in u2-os cells. using these cells, they ran the Fluxor™ assay to identify and WebFluxOR™ Differentially Identifies K+ Channel Antagonists Using FDSS6000 Reaction Time (sec) Z' Score FDSS7000EX Effect of reaction time on z' scores using hERG cells and … how far is georgetown from toronto

FluxOR Potassium Ion Channel Assay J1 13Aug2009

Cell-Based Potassium Ion Channel Screening Using …

WebSep 6, 2013 · For ATP depletion assays, H9C2 and RCM’s cells were plated in 96 well plates per the manufacturer’s protocol for 24 hr prior to treatments. For gene expression … WebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes advantage of the well described permeability of potassium channels to thallium (Tl+) ions.1,2 When thallium is added to the extracellular solution with a stimulus to open how far is georgetown from san antonioWebFluxOR™ thallium assay has been widely recognized for hERG activity test. This assay is optically based and can be performed on 96-well or 384-well plates as a homogenous assay for high-throughput screening (HTS) … how far is georgetown indiana from louisville

"WebThe Predictor™ hERG kit from Invitrogen is a homogeneous fluorescent assay that uses a simple add-and-read format. The assay is based on the principle of fluorescence … " - Fluxor herg assay h9c2

Fluxor herg assay h9c2

Cell-Based hERG Channel Inhibition Assay in High-Throughput …

Webs of hERG inhibitors determined in separate tests. N=4. FLIPR Potassium Kit-4 -2 0 2 0 1 3 Dofetilide Terfenadine Pimozide Log[inhibitor] mM) FluxOr Kit-4 -2 0 2 0.0 0.2 0.4 0.6 0.8 1.0 Dofetilide Terfenadine Pimozide Log[inhibitor] mM Compound) IC 50 (nM) FLIPR Potassium Assay 50) FluxOR Assay Dofetalide 39 53 Pimozide 135 195 Terfenadine … WebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 μL/well) and ...

Did you know?

WebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye … WebFluxOR™ Potassium Ion Channel Assayは、hERG を安定的に発現するCHO およびHEK293 細胞に使用できること、ならびに当社のBacMam カリウムチャネル標的試薬セットで導入可能なBacMam-hERG などのカリウムイオンチャネル遺伝子を一時

WebThe FluxOR Potassium Ion Channel Assay Kits ( F10016, F10017) provide a fluorescence-based assay for high-throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR Potassium Ion Channel Assay Kits take advantage of the well-described permeability of potassium channels to thallium (Tl +) ions. WebFluxxor - Home. Fluxxor is a set of tools to facilitate building JavaScript data layers using the Flux architecture by reifying many of the core Flux concepts. It works particularly well …

Webthe FluxOR™ II Green Potassium Ion Channel Assay (ThermoFisher Cat# F20016) and HEK293-hERG cells (human embryonic kidney cell line stably expressing hERG). … Webwater baths appear to act as hERG blockers and can reduce assay performance or cause complete assay failure. 4.1 Predictor™ hERG Tracer Red Store Predictor™ hERG …

WebApr 1, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2.Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for further …

WebMTT assay was carried out using H9c2 cells to determine a proper dose range for the designed assay. The ECG measurement revealed that intravenous injection of 4-AcO-DET at 2.0 mg/kg and 4-HO-MET at 1.5 mg/kg prolonged the QTc interval in SD rats. In addition, hERG potassium channels in Chinese hamster ovary cells treated test substances were ... how far is georgetown from my locationWebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye that results in a larger signal window and a significant improvement in … how far is georgetown texas from austin texasWebAfter the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by a thallium stimulus buffer addition (10 … how far is georgetown kyWebThree K+ Channels, hERG, Kir2.1, and Kv7.2, were differentially expressed in U-2 OS cells using BacMam technology. Following an overnight incubation cells were dye loaded using FluxOR then incubated with compounds or controls. Each of the four compound plates (Tocris) was tested against the three cell lines hERG, Kir2.1, and Kv7.2. high air forces customWebDec 15, 2010 · In the present study, we describe a high-capacity, 384- and 1,536-well plate, functional thallium flux assay for the hERG channel that fulfills these criteria. This assay was optimized and validated using different structural classes of hERG inhibitors. high air ground do you need a parentWebThe FluxOR™ II Green Potassium Ion Channel Assay is the next-generation solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR II Green assay takes … how far is georgetown ohio from meWebThe hERG assay, an in vitro electrophysiologic assay, has become routine since its development in the 1990s. It is one of the recommended studies in the ICH S7B regulatory guideline. This is a voltage clamp assay where a cultured mammalian cell (usually human embryonic kidney, or HEK cell) is transfected with hERG DNA. high air forces 1