WebNov 1, 2003 · The fission yeast strain T6, which expresses hsv-tk under control of the SV40 promoter was obtained from F. Antequera ( 21 ). This strain contains three to four copies of hsv-tk integrated in tandem into the leu1+ gene (F.Antequera, personal communication). 3 H-thymidine incorporation assay WebFeb 9, 2024 · Tetrad dissection is a powerful tool in yeast genetics that allows the analysis of products of a single meiosis. With just a few tetrads, it is possible to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days.
Fission yeast obeys a linear size law under nutrient titration
WebThe fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its … WebDec 6, 2016 · A crucial factor in maintaining genome stability is establishing deoxynucleoside triphosphate (dNTP) levels within a range that is optimal for chromosomal replication. Since DNA replication is relevant to a wide range of other chromosomal activities, these may all be directly or indirectly affected when dNTP concentrations … mike lackey lowes
General purpose tagging vectors for fission yeast - ScienceDirect
WebThe fission yeast is a single-celled fungus with simple, fully characterized genome and a rapid growth rate. It has long been used in brewing, baking, and molecular genetics. S. … WebApr 13, 2024 · The fission yeast, Schizosaccharomyces pombe exhibits the activation of several signal transduction pathways in response to various environmental conditions. WebSep 1, 2001 · The fission yeast has a symmetrical pattern of cell division and has been particularly popular for studies of cell growth and division, and chromosome dynamics. new westside park atlanta ga