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Mcherry-gfp-lc3质粒

Web（三）lc3双荧光自噬慢病毒原理 GFP是酸敏感型GFP蛋白，而mRFP是稳定的荧光表达基团，不受外界影响。 由于自噬小体进入第二阶段后，与溶酶体进行融合，形成自噬溶酶体。 WebHowever, mCherry-α-tubulin fusions were successfully incorporated into microtubules in most cells, similar to results seen with GFP-coupled tubulin. Shaner et al. (2004) This strongly suggests that the fluorescent protein tag caused clustering artifacts (Fig. 2a) and that ClpX and ClpP fluorescent protein fusions cannot be trusted for determining …

mCherry-EGFP-LC3B(Mouse)慢病毒滴度液-细胞自噬-产品中心-PPL质粒 …

WebHepG2 cells were transduced with mCherry-GFP-LC3 lentiviral vectors, which were pre-packed with the lentivirus packaging kit. The quenching properties of the GFP fluorescent signal in acid conditions (pH < 5) and the cherry fluorescent signal do not differ significantly in different acidic environments [ 28 ] when used to detect autophagic flux. http://www.biofeng.com/adjy/zhili/pBABE-puro%20mCherry-EGFP-LC3B.html church of pentecost maryland region https://prime-source-llc.com

pCMV-mCherry-p62(pCMV-mCherry-p62质粒)(D2818-1μg)

WebmCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with … WebDownload scientific diagram The GFP and mRFP signals of tfLC3 show different localization patterns. (A) Diagram of tfLC3 structure. (B) HeLa cells were cotransfected … WebPremo Autophagy sensors take advantage of autophagy-associated proteins LC3B or p62, along with GFP or RFP, to detect either the induction or inhibition of autophagy in cells. The Premo Autophagy sensors are transduced using BacMam (insect Bac ulovirus with a Mam malian promoter) technology, enabling unambiguous visualization of the chimeric ... dewar\u0027s japanese smooth 8 year whisky

自噬研究_自噬单双标腺病毒/腺相关病毒｜维真生物

Category:mCherry :: Fluorescent Protein Database

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Mcherry-gfp-lc3质粒

pCMV-GFP-LC3B(pCMV-GFP-LC3B质粒)(D2815-1μg)

Web28 jun. 2016 · H4 mCherry-GFP-LC3 and H4 mCherry-GFP-UFM1 lines were generated by lentiviral delivery of mCherry-GFP-LC3 and mCherry-GFP-UFM1 in pLenti6.3 followed by selection for stable integration with blasticidin. Coding sequences for MAP1LC3B and UFM1 were derived from Invitrogen entry clones (IOH13267, IOH7279). WebFigure 1A, the detection of mCherry-GFP-LC3-positive dots and the measurement of autophagy flux was reproducible and sensitive, and LC3 was easily detected with different microscopy methods, including direct epifluorescence, confocal microscopy, a super zoom microscope, and spinning-disk microscopy. Using this combinatorial strategy, a robust LC3

Mcherry-gfp-lc3质粒

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WebA p27 +/+ and p27 −/− mCherry-eGFP LC3 MEFs were transfected with siRNA against ATAT1 or control siRNA. After 24 h, cells were starved for either glucose or aa for further 24 h. Scale bar 50 µm. Web7 nov. 2015 · Both clones expressed 75kDa mCherry-EGFP-LC3 fusion protein, while clone relativelylow expression level (Fig. 1C). faintband 25kDa detected westernblots may …

WebPlasmid pSIN-PAmCherry-KFERQ-NE from Dr. Shu Leong Ho's lab contains the inserts PA-mCherry and KFERQ peptide and is published in Autophagy. 2024 Apr 14:1-24. doi: 10.1080/15548627.2024.1603545. ... pMRX-IP-GFP-LC3-RFP-LC3ΔG Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. WebpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染细胞后能够在靶细胞中高效表达绿色荧光蛋白GFP标签的LC3B的融合蛋白，呈现明亮的绿色荧光，可以用于细胞自噬(autophagy)的研究。

WebTo explain our findings, GFP-LC3 and GFP-RFP-LC3 adenoviruses and autophagy regulators were used, and the results suggested that doxazosin blocked autophagy in HSCs in vitro. To further confirm our findings, an mCherry-EGFP-LC3 adeno-associated virus was administered to mice, and the results showed that doxazosin suppressed autophagy in … WebC3003-100μl. Lenti-EGFP-LC3B (10^9TU/ml) 100μl. 2992.00元. Lenti-EGFP-LC3B，即Lentivirus expressing EGFP-LC3B fusion protein，是一种可以表达EGFP-LC3B融合蛋白的慢病毒，可以用于感染细胞或组织后进行细胞自噬 (autophagy)检测。. 本产品带有嘌呤霉素 (puromycin)抗性，感染细胞后可以筛选 ...

Web自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。 当自噬溶酶体形成后，酸性环境使GFP荧光淬灭， …

Web细胞自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至细胞自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。 当细胞自噬溶酶体形成后，酸性的溶酶体环境使GFP荧光淬灭，而mCherry荧光不受影响，细胞自噬溶酶体呈现红色荧光（图6）。 church of pentecost md facebookWebPlasmid pDEST-CMV mCherry-GFP-LC3B WT from Dr. Robin Ketteler's lab contains the insert Microtubule-associated proteins 1A/1B light chain 3B and is published in … church of pentecost manchesterWebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry标签的p62融合蛋白，呈现明亮的红色荧光，可以用于细胞自噬(autophagy)的研究 church of pentecost mdWebpBABE-puro mCherry-EGFP-LC3B质粒价格1200元，pBABE-puro mCherry-EGFP-LC3B(爱迪基因#22418 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为氨苄青霉素，质粒大小为5169 bp。pBABE-puro mCherry-EGFP-LC3B质粒是哺乳动物表达，逆转 … dewar\u0027s japanese smooth priceWebPlasmid pBABE-puro mCherry-EGFP-LC3B from Dr. Jayanta Debnath's lab contains the insert microtubule-associated protein 1 light chain 3 beta and is published in EMBO Rep. … church of pentecost marylandWebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表 … church of pentecost logosWeb17 feb. 2024 · In stable RFP-GFP-LC3 U87 and PC-12 cells, ethyl ... JC as revealed by the colocalization of MitoTracker with GFP-LC3 puncta and the decreased GFP/RFP ratio illuminated by mCherry-GFP-FIS1101 ... church of pentecost milton keynes