Phospholipidosis assay

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August undefined, 2024

WebThe LipidTOX assay series includes neutral lipid stains for fixed cells and phospholipid stains for live cells, which can be combined in multiplexed assays. Automatically … WebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high …

Development of acidic phospholipid containing immobilized artificial …

WebDec 17, 2016 · Drug-induced phospholipidosis caused by combinations of common drugs in vitro.药物引起phospholipidosis引起的常见药物在体外 ... Assay protocol phospholipidosisinduction, H4 cells were plated NuncF96 MicroWell white polystyrene plates (Nunc, Langenselbold, Germany) cellsper well mediumeach wereincubated … WebPhospholipidosis (PL) is a phospholipid storage disorder, resulting in excessive accumulation of phospholipids in lysosomes of various tissue types [199, 200]. From:Progress in Medicinal Chemistry, 2011 Related terms: Amiodarone Patient Phospholipid Storage Disease Lysosome Storage Disease Niemann Pick Disease … irish teacher gets arrested pronouns

doi: 10.1007/978-1-0716-2213-1 8 - ResearchGate

WebeCiphr ® Cardio — iPS cell-derived cardiomyocytes and MEA. Structural cardiotoxicity assay — using 3D spontaneously beating microtissues. Combined hypertrophy and cardiotoxicity assay — using 3D spontaneously beating microtissues. Cardiotox screen — … WebDec 1, 2011 · In summary, we demonstrated an in vitro phospholipidosis screening assay in CHO-K1 and HepG2 cells based on a high content screening analysis method that can … WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. … port for lung cancer

Adaptation of an In Vitro Phospholipidosis Assay to an Automated …

In Silico Assay for Assessing Phospholipidosis Potential …

WebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a … WebJun 21, 2024 · 1. Introduction. Drug-induced phospholipidosis (DIPLD) is characterized by excessive intracellular accumulation of phospholipids as a result of drug treatments [1].Since the first DIPLD case reported in 1948 [2], an increasing number of drugs were identified as PLD inducers, including antibiotics, antidepressants, antipsychotics and so … irish teaching councilWebMar 5, 2015 · Synthesis and phospholipidosis effect of a series of cationic amphiphilic compounds: a case study to evaluate in silico and in vitro assays Article Full-text available port for lifesteal smp

"WebFeb 1, 2007 · Phospholipidosis was determined by algorithms that evaluated the granular texture of the perinuclear area as an indicator of the amount of phospholipidosis vacuoles. ... High-content screening... " - Phospholipidosis assay

Phospholipidosis assay

Development of acidic phospholipid containing immobilized artificial …

WebTraductions en contexte de "monitored by assay" en anglais-français avec Reverso Context : If self-injection with Improvac is suspected, reproductive physiology should be monitored by assay of testosterone or oestrogen levels (as appropriate). The risk WebMembrane permeability assays demonstrate that the alkaloids are biologically available, although fangchinoline showed lower permeability than tetrandrine. At high concentrations, the extract, alkaloid fractions, and pure alkaloids induced phospholipidosis in 293TAT cells and less so in VeroAT cells. Gene expression profiling during virus ...

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WebNational Center for Biotechnology Information WebMar 24, 2024 · Drug-induced phospholipidosis: Human: ASCs: Matrigel: Expression of CYP450 enzyme (CYP3A4); ... (LoT) assay to analyze the hepatoxicity of 238 marketed drugs (32 negative controls and 206 reported DILI agents) and showed high predictive values of the LoT system with 88.7% sensitivity and 88.9% specificity.

WebJun 22, 2024 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established … WebinstaCELL® Phospholipidosis Assay Kits. Drug induced accumulation of phospholipids (phospholipidosis) is associated with clinical toxicities. Cell‐based assays can help to …

WebIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H 2 …

WebMar 1, 2024 · This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds...

WebTo identify compounds that induce intracellular phospholipidosis, we used a high-content imaging method to optimize and validate the cell-based PLD assay. The assay was … irish tea vs english teaWebEchelon’s LPLA2 Inhibitor Screen is a high throughput assay designed to screen drugs abilitiy to inhibit LPLA2 activity in vitro, a potential predictor of drug-induced phospholipidosis. Sample Volume: 1 μL. Sample Number: 128 Samples (in triplicates)/384-well plate. Echelon’s LPLA2 Inhibitor Screen (K-7000I) is designed to assay a drug’s ... port for home theaterWebJul 2, 2008 · In vitro screening assays were developed in HepG2 cells, rat primary hepatocytes, and rhesus monkey hepatocytes using the fluorescent-labeled phospholipid probe N - (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (NBD-PE) or Nile Red lipid stain. port for mapping network driveWebSuch assays rely heavily on the development prospective assays aim to predict toxicities that could lead to the ter- of human physiologically relevant model systems in combination with mination of a drug candidate or an entire development programme, appropriate toxicity end points. and bespoke retrospective assays are used for issue resolution ... irish teacher murderedWebcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a port for lymphomaWebCyprotex offer a number of other services including reactive metabolite detection using trapping agents, a cell stress panel, drug-induced phospholipidosis and steatosis, lysosomal trapping, hemolysis, mitochondrial toxicity (mitochondrial biogenesis assay, Glu/Gal assay, functional mitochondrial toxicity assay and the mitochondrial respiratory ... irish teaching council registrationWebFeb 1, 2006 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established NBD-PE staining assay... irish teacher in prison